| First Author | Millar JC | Year | 2015 |
| Journal | Invest Ophthalmol Vis Sci | Volume | 56 |
| Issue | 10 | Pages | 5764-76 |
| PubMed ID | 26325415 | Mgi Jnum | J:230937 |
| Mgi Id | MGI:5766568 | Doi | 10.1167/iovs.15-16720 |
| Citation | Millar JC, et al. (2015) Strain and Age Effects on Aqueous Humor Dynamics in the Mouse. Invest Ophthalmol Vis Sci 56(10):5764-76 |
| abstractText | PURPOSE: We evaluated differences in aqueous humor dynamics (AHD) among several mouse strains within younger and older age groups. METHODS: Albino (A/J, BALB/cJ) and pigmented (C3H/HeJ, C57-BL/6J) mice (young [2(1/2)-4(1/2) months] and aged [10-12 months]) were studied. Intraocular pressure (IOP) was measured. In cannulated eyes, episcleral venous pressure (Pe) was assessed (blood reflux). Other AHD parameters (outflow facility [C], aqueous humor formation rate [Fin]) were assessed (constant flow infusion). Uveoscleral outflow rate (Fu) was obtained by calculation (Fu(calc)) using the modified Goldmann equation, and in additional eyes (for comparison), by FITC-dextran perfusion (Fu(FITC-dex)). RESULTS: Intraocular pressure was higher in pigmented strains, but did not exhibit age-dependence, except in the C57-BL/6J strain. Fu(calc) decreased with age in BALB/cJ ( downward arrow83.3%), C3H/HeJ ( downward arrow78.0%), and C57-BL/6J ( downward arrow85.0%) strains. In the A/J strain, Fu(calc) decreased with age ( downward arrow70.0%), but not significantly. Fin decreased with age in the C3H/HeJ ( downward arrow53.6%) strain. In C57-BL/6J and A/J strains, Fin decreased with age, but not significantly. C in the BALB/cJ strain increased with age ( upward arrow62.5%). In C3H/HeJ and C57-BL/6J strains, C increased with age, but not significantly. Episcleral venous pressure ranged from 6.0 to 6.6 mm Hg (albino strains) to 8.5 to 8.9 mm Hg (pigmented strains). Pe was not age dependent, but was higher in pigmented animals. CONCLUSIONS: In mouse, Fu and Fin diminish with age. C tends to increase as animals progress to middle life. There are strain differences in Fu, IOP, C, Fin, and Pe. The current findings provide an important foundation for comparisons among different strains in different study reports. |
