| First Author | Jun YW | Year | 2016 |
| Journal | FEBS Lett | Volume | 590 |
| Issue | 7 | Pages | 954-61 |
| PubMed ID | 26950402 | Mgi Jnum | J:231918 |
| Mgi Id | MGI:5775500 | Doi | 10.1002/1873-3468.12123 |
| Citation | Jun YW, et al. (2016) D-AKAP1a is a signal-anchored protein in the mitochondrial outer membrane. FEBS Lett 590(7):954-61 |
| abstractText | Dual A-kinase anchoring protein 1a (D-AKAP1a, AKAP1) regulates cAMP signaling in mitochondria. However, it is not clear how D-AKAP1a is associated with mitochondria. In this study, we show that D-AKAP1a is a transmembrane protein in the mitochondrial outer membrane (MOM). We revealed that the N-terminus of D-AKAP1a is exposed to the intermembrane space of mitochondria and that its C-terminus is located on the cytoplasmic side of the MOM. Moderate hydrophobicity and the positively charged flanking residues of the transmembrane domain of D-AKAP1a were important for targeting. Taken together, D-AKAP1a can be classified as a signal-anchored protein in the MOM. Our topological study provides valuable information about the molecular and cellular mechanisms of mitochondrial targeting of AKAP1. |
