| First Author | Yu LT | Year | 2016 |
| Journal | Mol Cell Endocrinol | Volume | 422 |
| Pages | 150-159 | PubMed ID | 26683606 |
| Mgi Jnum | J:237097 | Mgi Id | MGI:5810862 |
| Doi | 10.1016/j.mce.2015.12.002 | Citation | Yu LT, et al. (2016) Recombinant Reg3alpha protein protects against experimental acute pancreatitis in mice. Mol Cell Endocrinol 422:150-9 |
| abstractText | Regenerating gene 3alpha (Reg3alpha) protein is a trophic factor that stimulates cell and tissue proliferation, neogenesis and also acts against apoptosis and necrosis. In order to explore the potential roles of recombinant Reg3alpha (rReg3alpha), we produced a mature rReg3alpha polypeptide for direct administration in l-arginine (L-Arg) induced acute pancreatitis (AP) in mice. Our results showed that rReg3alpha stimulated cell proliferation through Erk1/2 and p38 phosphorylation and also cyclin D1 upregulation mediated by Akt/ATF-2 signaling. Moreover, rReg3alpha administration significantly reduced the pancreatic damage caused by L-Arg injection, as shown in histological examination and serum amylase, lipase and C-reactive protein (CRP) assays. Not only acinar cell necrosis but also apoptosis found in the pancreas of AP mice were alleviated by rReg3alpha. Finally, upregulated Bcl-2 and Bcl-xL and suppressed poly (ADP-ribose) synthetase/polymerase (PARP) levels were detected as being relevant to the mechanism of rReg3alpha protection. We therefore conclude that rReg3alpha acts as a protective polypeptide against AP in mice by enhancing Bcl-2 and Bcl-xL expressions and suppressing PARP level. |
