| First Author | Chen J | Year | 2016 |
| Journal | Neurosci Lett | Volume | 626 |
| Pages | 79-85 | PubMed ID | 27222923 |
| Mgi Jnum | J:241465 | Mgi Id | MGI:5902669 |
| Doi | 10.1016/j.neulet.2016.05.031 | Citation | Chen J, et al. (2016) MicroRNA-128-3p impaired water maze learning by suppressing Doublecortin expression in both wild type and Abeta-42 infused mice. Neurosci Lett 626:79-85 |
| abstractText | MicroRNA-128-3p (miR-128) is a brain-enriched microRNA reported to target Doublecortin (Dcx), a key transcriptional factor during adult neurogenesis. However, the downstream physiological effects of this miR-128-DCX axis remain unclear. Here we demonstrated that miR-128 could suppress Dcx expression by complementally binding to the -849 to -856 region of the 3'UTR of mouse Dcx. During differentiation of neural stem cells, over-expressing miR-128 with a lentivirus system inhibited the up-regulation of Dcx on Day 5, subsequently decreasing the percentage of TuJ+ cells on Day 16. Administration of the lentivirus encoding miR-128 into mouse hippocampi significantly impaired water maze learning after 14days, which could be attenuated when the Dcx-encoding virus was delivered simultaneously. In addition, similar changes including miR-128 up-regulation, Dcx down-regulation and learning defects were observed after a 14-day infusion of Abeta-42, which were also partially reversed by over-expressing Dcx. Collectively, the regulation axis from miR-128 to Dcx is critical for hippocampus-related contextual learning not only in wild type, but also in mice infused with Abeta-42. |
