| First Author | Jacovetti C | Year | 2024 |
| Journal | Mol Metab | Volume | 84 |
| Pages | 101955 | PubMed ID | 38704026 |
| Mgi Jnum | J:359769 | Mgi Id | MGI:7642467 |
| Doi | 10.1016/j.molmet.2024.101955 | Citation | Jacovetti C, et al. (2024) The mitochondrial tRNA-derived fragment, mt-tRF-Leu(TAA), couples mitochondrial metabolism to insulin secretion. Mol Metab 84:101955 |
| abstractText | OBJECTIVE: The contribution of the mitochondrial electron transfer system to insulin secretion involves more than just energy provision. We identified a small RNA fragment (mt-tRF-Leu(TAA)) derived from the cleavage of a mitochondrially-encoded tRNA that is conserved between mice and humans. The role of mitochondrially-encoded tRNA-derived fragments remains unknown. This study aimed to characterize the impact of mt-tRF-Leu(TAA), on mitochondrial metabolism and pancreatic islet functions. METHODS: We used antisense oligonucleotides to reduce mt-tRF-Leu(TAA) levels in primary rat and human islet cells, as well as in insulin-secreting cell lines. We performed a joint transcriptome and proteome analysis upon mt-tRF-Leu(TAA) inhibition. Additionally, we employed pull-down assays followed by mass spectrometry to identify direct interactors of the fragment. Finally, we characterized the impact of mt-tRF-Leu(TAA) silencing on the coupling between mitochondrial metabolism and insulin secretion using high-resolution respirometry and insulin secretion assays. RESULTS: Our study unveils a modulation of mt-tRF-Leu(TAA) levels in pancreatic islets in different Type 2 diabetes models and in response to changes in nutritional status. The level of the fragment is finely tuned by the mechanistic target of rapamycin complex 1. Located within mitochondria, mt-tRF-Leu(TAA) interacts with core subunits and assembly factors of respiratory complexes of the electron transfer system. Silencing of mt-tRF-Leu(TAA) in islet cells limits the inner mitochondrial membrane potential and impairs mitochondrial oxidative phosphorylation, predominantly by affecting the Succinate (via Complex II)-linked electron transfer pathway. Lowering mt-tRF-Leu(TAA) impairs insulin secretion of rat and human pancreatic beta-cells. CONCLUSIONS: Our findings indicate that mt-tRF-Leu(TAA) interacts with electron transfer system complexes and is a pivotal regulator of mitochondrial oxidative phosphorylation and its coupling to insulin secretion. |
