| First Author | Hao X | Year | 2017 |
| Journal | FEBS Lett | Volume | 591 |
| Issue | 8 | Pages | 1114-1125 |
| PubMed ID | 28337758 | Mgi Jnum | J:243308 |
| Mgi Id | MGI:5908077 | Doi | 10.1002/1873-3468.12631 |
| Citation | Hao X, et al. (2017) Novel immunoassays to detect methionine adenosyltransferase activity and quantify S-adenosylmethionine. FEBS Lett 591(8):1114-1125 |
| abstractText | We present a novel real-time immunoassay to measure methionine adenosyltransferase (MAT) activity that integrates the MAT-catalyzed reaction of Met and adenosine triphosphate to produce S-adenosylmethionine (SAM) and a highly sensitive immunoassay to specifically quantify SAM simultaneously. The cellular localization of SAM and S-adenosylhomocysteine varies with cell proliferation status: in normal cells, they are found mostly in the cytoplasm, but localize to the nucleus in proliferating cells. MAT-I/III activity is stimulated by Met, but inhibited by S-nitrosoglutathione, and the methylation index (MI) increases after Met stimulation of L02 cells. Met and S-nitrosoglutathione inhibit MAT-II activity, and the MI decreases after Met stimulation of HepG2 cells. The method described provides a significant advancement in the field for the measurement of MAT activity under various conditions. |
