| First Author | Balboula AZ | Year | 2017 |
| Journal | Biol Reprod | Volume | 96 |
| Issue | 6 | Pages | 1197-1209 |
| PubMed ID | 28575288 | Mgi Jnum | J:247937 |
| Mgi Id | MGI:5918873 | Doi | 10.1093/biolre/iox047 |
| Citation | Balboula AZ, et al. (2017) Maternal RNA regulates Aurora C kinase during mouse oocyte maturation in a translation-independent fashion. Biol Reprod 96(6):1197-1209 |
| abstractText | During oocyte meiotic maturation, Aurora kinase C (AURKC) is required to accomplish many critical functions including destabilizing erroneous kinetochore-microtubule (K-MT)attachments and regulating bipolar spindle assembly. How localized activity of AURKC is regulated in mammalian oocytes, however, is not fully understood. Female gametes from many species, including mouse, contain stores of maternal transcripts that are required for downstream developmental events. We show here that depletion of maternal RNA in mouse oocytes resulted in impaired meiotic progression, increased incidence of chromosome misalignment and abnormal spindle formation at metaphase I (Met I), and cytokinesis defects. Importantly, depletion of maternal RNA perturbed the localization and activity of AURKC within the chromosomal passenger complex (CPC). These perturbations were not observed when translation was inhibited by cycloheximide (CHX) treatment. These results demonstrate a translation-independent function of maternal RNA to regulate AURKC-CPC function in mouse oocytes. |
