| First Author | Bae IS | Year | 2017 |
| Journal | Gene | Volume | 626 |
| Pages | 64-69 | PubMed ID | 28495174 |
| Mgi Jnum | J:246413 | Mgi Id | MGI:5920941 |
| Doi | 10.1016/j.gene.2017.05.016 | Citation | Bae IS, et al. (2017) PPARgamma-mediated G-protein coupled receptor 120 signaling pathway promotes transcriptional activation of miR-143 in adipocytes. Gene 626:64-69 |
| abstractText | MicroRNAs (miRNAs), the small noncoding RNAs, regulate various biological processes such as adipogenesis. MicroRNA-143 (miR-143) promotes adipocyte differentiation, and is correlated with obesity in mice fed a high-fat diet. However, the transcriptional regulation of miR-143 is largely unknown. In this study, we identified that miR-143 is a target of peroxisome proliferator-activated receptor gamma (PPARgamma), a key transcription factor in adipogenesis. Four putative peroxisome proliferator response elements (PPREs) were identified in the miR-143 promoter region. Using chromatin immune-precipitation, we observed that PPARgamma was bound with two PPRE regions of the miR-143 promoter in 3T3-L1 adipocytes. A luciferase reporter assay showed that the PPRE1 region (-1330/-1309) of the miR-143 promoter played an important role in PPARgamma transcriptional activation. In addition, we determined that G-protein coupled receptor 120 (GPR 120), which functions as an omega 3 fatty acid receptor, affected miR-143 expression in adipocytes. GPR120 silencing in adipocytes inhibited the expression of PPARgamma and miR-143, whereas GPR120 overexpression led to increased expressions of PPARgamma and miR-143. Silencing of PPARgamma inhibited the induction of miR-143 by GPR-120. These results suggested that a PPARgamma-mediated GPR120 signaling pathway promotes transcriptional activation of miR-143 in adipocytes. |
