| First Author | Cisternas CD | Year | 2017 |
| Journal | Sci Rep | Volume | 7 |
| Issue | 1 | Pages | 5320 |
| PubMed ID | 28706210 | Mgi Jnum | J:252889 |
| Mgi Id | MGI:5927129 | Doi | 10.1038/s41598-017-05658-6 |
| Citation | Cisternas CD, et al. (2017) Regulation of aromatase expression in the anterior amygdala of the developing mouse brain depends on ERbeta and sex chromosome complement. Sci Rep 7(1):5320 |
| abstractText | During development sex differences in aromatase expression in limbic regions of mouse brain depend on sex chromosome factors. Genes on the sex chromosomes may affect the hormonal regulation of aromatase expression and this study was undertaken to explore that possibility. Male E15 anterior amygdala neuronal cultures expressed higher levels of aromatase (mRNA and protein) than female cultures. Furthermore, treatment with oestradiol (E2) or dihydrotestosterone (DHT) increased Cyp19a1 expression and aromatase protein levels only in female neuronal cultures. The effect of E2 on aromatase expression was not imitated by oestrogen receptor (ER) alpha agonist PPT or the GPER agonist G1, but it was fully reproduced by DPN, a specific ligand of ERbeta. By contrast, the effect of DHT on aromatase expression was not blocked by the anti-androgen flutamide, but completely abrogated by the ERbeta antagonist PHTPP. Experiments using the four core genotype model showed a sex chromosome effect in ERbeta expression (XY > XX) and regulation by E2 or DHT (only XX respond) in amygdala neurons. In conclusion, sex chromosome complement governs the hormonal regulation of aromatase expression through activation of ERbeta in developing mouse brain. |
