| First Author | Zhao JJ | Year | 2016 |
| Journal | Arch Biochem Biophys | Volume | 604 |
| Pages | 27-35 | PubMed ID | 27267730 |
| Mgi Jnum | J:248837 | Mgi Id | MGI:6093151 |
| Doi | 10.1016/j.abb.2016.06.002 | Citation | Zhao JJ, et al. (2016) Dihydrocapsaicin suppresses proinflammatory cytokines expression by enhancing nuclear factor IA in a NF-kappaB-dependent manner. Arch Biochem Biophys 604:27-35 |
| abstractText | BACKGROUND: Atherosclerosis is a chronic inflammatory disease and represents the leading cause of morbidity and mortality throughout the world. Accumulating evidences have showed that Dihydrocapsaicin (DHC) has been found to exert multiple pharmacological and physiological effects. Nevertheless, the effects and possible mechanism of DHC on proinflammatory response remain largely unexplained. METHODS AND RESULTS: We found that DHC markedly upregulated NFIA and suppressed NF-kappaB expression in THP-1 macrophages. Up-regulation of proinflammatory cytokines induced by LPS including TNF-alpha, IL-1beta and IL-6 were markedly suppressed by DHC treatment. We also observed that protein level of NFIA was significantly increased while NF-kappaB and proinflammatory cytokines were decreased by DHC treatment in apoE(-/-) mice. Lentivirus-mediated overexpression of NFIA suppressed NF-kappaB and proinflammatory cytokines expression both in THP-1 macrophages and plaque tissues of apoE-/- mice. Moreover, treatment with lentivirus-mediated overexpression of NFIA made the down-regulation of DHC on NF-kappaB and proinflammatory cytokines expression notably accentuated in THP-1 macrophages and apoE(-/-) mice. In addition, treatment with siRNA targeting NF-kappaB accentuated the suppression of proinflammatory cytokines by lentivirus-mediated overexpression of NFIA. CONCLUSION: These observations demonstrated that DHC can significantly decrease proinflammatory cytokines through enhancing NFIA and inhibiting NF-kappaB expression and thus DHC may be a promising candidate as an anti-inflammatory drug for atherosclerosis as well as other disorders. |
