| First Author | Laszkiewicz A | Year | 2014 |
| Journal | PLoS One | Volume | 9 |
| Issue | 9 | Pages | e106927 |
| PubMed ID | 25198102 | Mgi Jnum | J:254589 |
| Mgi Id | MGI:6112371 | Doi | 10.1371/journal.pone.0106927 |
| Citation | Laszkiewicz A, et al. (2014) Ikaros and RAG-2-mediated antisense transcription are responsible for lymphocyte-specific inactivation of NWC promoter. PLoS One 9(9):e106927 |
| abstractText | Recombination activating gene-2 (RAG-2) and NWC are strongly evolutionarily conserved overlapping genes which are convergently transcribed. In non-lymphoid cells the NWC promoter is active whereas in lymphocytes it is inactive due to the DNA methylation. Analysing the mechanism responsible for lymphocyte-specific methylation and inactivation of NWC promoter we found that Ikaros, a lymphocyte-specific transcription factor, acts as a repressor of NWC promoter--thus identifying a new Ikaros target--but is insufficient for inducing its methylation which depends on the antisense transcription driven by RAG-2 promoter. Possible implications of these observations for understanding evolutionary mechanisms leading to lymphocyte specific expression of RAG genes are discussed. |
