| First Author | McDermott AJ | Year | 2018 |
| Journal | Immunology | Volume | 153 |
| Issue | 4 | Pages | 513-522 |
| PubMed ID | 29055116 | Mgi Jnum | J:259201 |
| Mgi Id | MGI:6120168 | Doi | 10.1111/imm.12853 |
| Citation | McDermott AJ, et al. (2018) Inhaled Cryptococcus neoformans elicits allergic airway inflammation independent of Nuclear Factor Kappa B signalling in lung epithelial cells. Immunology 153(4):513-522 |
| abstractText | Pulmonary challenge with the ubiquitous fungus Cryptococcus neoformans results in allergic airway inflammation (AAI) characterized by robust recruitment of eosinophils and T cells producing type 2 cytokines to the lungs. Previous studies have demonstrated a critical role for Nuclear Factor Kappa B (NF-kappaB) activation within lung epithelial cells (LECs) in driving AAI in response to protein allergens, yet the role of LEC-intrinsic NF-kappaB in promoting AAI following exposure to C. neoformans is poorly understood. To investigate the role of LEC-intrinsic NF-kappaB in promoting AAI following C. neoformans challenge, we used IKK()(LEC) mice, which lack canonical NF-kappaB activation specifically within LECs. IKK()(LEC) and littermate control mice were intranasally challenged with 10(6) CFU of C. neoformans strain 52D, and lung tissues were collected at 7, 14 and 21 days post infection to assess the development of AAI. Notably, the absence of epithelial NF-kappaB signalling did not affect the magnitude or kinetics of lung eosinophilia when compared with the response in wild-type control mice. The total numbers of lung T cells producing the type 2 cytokines interleukin-5 and interleukin-13 were also unchanged in IKK()(LEC) mice. Furthermore, IKK()(LEC) mice showed no defect in the recruitment of protective interferon-gamma-producing CD4 T cells to the lungs, fungal clearance, or host survival compared with control mice. Immunofluorescence imaging surprisingly revealed no evidence of nuclear localization of NF-kappaB in LECs in response to C. neoformans challenge, indicating that NF-kappaB is not activated within these cells. Taken together, these data strongly suggest that NF-kappaB signalling within LECs does not promote AAI observed in response to C. neoformans. |
