| First Author | Pantaleão L | Year | 2018 |
| Journal | Exp Cell Res | Volume | 367 |
| Issue | 2 | Pages | 282-290 |
| PubMed ID | 29649428 | Mgi Jnum | J:268409 |
| Mgi Id | MGI:6271180 | Doi | 10.1016/j.yexcr.2018.04.008 |
| Citation | Pantaleao L, et al. (2018) Connections of annexin A1 and translocator protein-18kDa on toll like receptor stimulated BV-2 cells. Exp Cell Res 367(2):282-290 |
| abstractText | BACKGROUND: Annexin A1 (ANXA1) and Translocator Protein-18KDa (TSPO) down-regulate neuroinflammation. We investigated the role of recombinant ANXA1 (rANXA) on TSPO functions on Toll Like Receptor (TLR) activated microglia. METHODS: BV-2 cells (murine microglia), were stimulated by E. coli Lipopolysaccharide (LPS) and treated with rANXA1 in order to measure TSPO expression and inflammatory parameters. Anti-sense ANXA1 and TLR4 and TSPO shRNA, as well as pharmacological treatments, were employed to assess the mechanisms involved. RESULTS: LPS-stimulated BV-2 cells caused overexpression of TSPO, which was inhibited by: pharmacological blockade of TLR4 or TLR4 mRNA silencing; inhibition of myeloid differentiation primary response gene 88 (MyD88) dimerization; or blocking of nuclear factor kappaB (NF-kappaB) activation. rANXA1 treatment impaired LPS-induced TSPO upregulation by down-modulating MyD88 and NF-kappaB signaling; the effect was abolished by WRW4, an antagonist of formyl peptide receptor 2 (FPR2). rANXA1 treatment also downregulated interleukin 1beta (IL-1beta) and tumor necrosis factor-alpha (TNFalpha) secretion in LPS-stimulated BV-2 cells. TSPO knockdown in BV-2 cells augmented LPS-induced TNFalpha secretion and abolished the inhibitory effect of rANXA1 on TNFalpha secretion evoked by LPS. CONCLUSIONS: exogenous ANXA1 down-modulates LPS-induced TSPO via MyD-88/NF-kappaB pathways, and constitutive TSPO is pivotal for the control of ANXA1 on TNFalpha secretion. TSPO actions may be involved with the mechanisms of ANXA1 on inflammatory brain diseases. |
