| First Author | Hai L | Year | 2019 |
| Journal | Genesis | Volume | 57 |
| Issue | 3 | Pages | e23281 |
| PubMed ID | 30628160 | Mgi Jnum | J:273346 |
| Mgi Id | MGI:6285357 | Doi | 10.1002/dvg.23281 |
| Citation | Hai L, et al. (2019) Using CRISPR/Cas9 engineering to generate a mouse with a conditional knockout allele for the promyelocytic leukemia zinc finger transcription factor. Genesis 57(3):e23281 |
| abstractText | The promyelocytic leukemia zinc finger (PLZF) transcription factor mediates a wide-range of biological processes. Accordingly, perturbation of PLZF function results in a myriad of physiologic defects, the most conspicuous of which is abnormal skeletal patterning. Although whole body knockout of Plzf in the mouse (Plzf (KO) ) has significantly expanded our understanding of Plzf function in vivo, a conditional knockout mouse model that enables tissue or cell-type specific ablation of Plzf has not been developed. Therefore, we used CRISPR/Cas 9 gene editing to generate a mouse model in which exon 2 of the murine Plzf gene is specifically flanked (or floxed) by LoxP sites (Plzf (f/f) ). Crossing our Plzf (f/f) mouse with a global cre-driver mouse to generate the Plzf (d/d) bigenic mouse, we demonstrate that exon 2 of the Plzf gene is ablated in the Plzf (d/d) bigenic. Similar to the previously reported Plzf (KO) mouse, the Plzf (d/d) mouse exhibits a severe defect in skeletal patterning of the hindlimb, indicating that the Plzf (f/f) mouse functions as designed. Therefore, studies in this short technical report demonstrate that the Plzf (f/f) mouse will be useful to investigators who wish to explore the role of the Plzf transcription factor in a specific tissue or cell-type. |
