| First Author | Kolliopoulos C | Year | 2019 |
| Journal | J Biol Chem | Volume | 294 |
| Issue | 11 | Pages | 4119-4136 |
| PubMed ID | 30622137 | Mgi Jnum | J:275967 |
| Mgi Id | MGI:6307421 | Doi | 10.1074/jbc.RA118.004984 |
| Citation | Kolliopoulos C, et al. (2019) Transforming growth factor beta (TGFbeta) induces NUAK kinase expression to fine-tune its signaling output. J Biol Chem 294(11):4119-4136 |
| abstractText | TGFbeta signaling via SMAD proteins and protein kinase pathways up- or down-regulates the expression of many genes and thus affects physiological processes, such as differentiation, migration, cell cycle arrest, and apoptosis, during developmental or adult tissue homeostasis. We here report that NUAK family kinase 1 (NUAK1) and NUAK2 are two TGFbeta target genes. NUAK1/2 belong to the AMP-activated protein kinase (AMPK) family, whose members control central and protein metabolism, polarity, and overall cellular homeostasis. We found that TGFbeta-mediated transcriptional induction of NUAK1 and NUAK2 requires SMAD family members 2, 3, and 4 (SMAD2/3/4) and mitogen-activated protein kinase (MAPK) activities, which provided immediate and early signals for the transient expression of these two kinases. Genomic mapping identified an enhancer element within the first intron of the NUAK2 gene that can recruit SMAD proteins, which, when cloned, could confer induction by TGFbeta. Furthermore, NUAK2 formed protein complexes with SMAD3 and the TGFbeta type I receptor. Functionally, NUAK1 suppressed and NUAK2 induced TGFbeta signaling. This was evident during TGFbeta-induced epithelial cytostasis, mesenchymal differentiation, and myofibroblast contractility, in which NUAK1 or NUAK2 silencing enhanced or inhibited these responses, respectively. In conclusion, we have identified a bifurcating loop during TGFbeta signaling, whereby transcriptional induction of NUAK1 serves as a negative checkpoint and NUAK2 induction positively contributes to signaling and terminal differentiation responses to TGFbeta activity. |
