| First Author | Kadoyama K | Year | 2019 |
| Journal | Neurosci Lett | Volume | 712 |
| Pages | 134484 | PubMed ID | 31505240 |
| Mgi Jnum | J:282752 | Mgi Id | MGI:6378929 |
| Doi | 10.1016/j.neulet.2019.134484 | Citation | Kadoyama K, et al. (2019) Changes in the expression of prefoldin subunit 5 depending on synaptic plasticity in the mouse hippocampus. Neurosci Lett 712:134484 |
| abstractText | Prefoldin is a molecular chaperone that assists the folding of newly synthesized polypeptide chains and prevents aggregation of misfolded proteins. Dysfunction of prefoldin is one of the causes of neurodegenerative diseases such as Alzheimer's disease. The aim of this study was to clarify the involvement of prefoldin subunit 5 (PFDN5) in synaptic plasticity. PFDN5 protein expressed in the hippocampus was predominantly localized in the pyramidal cell layer of CA1-CA3 regions. Nicotine application caused a long-term potentiation (LTP)-like facilitation in vivo, that is synaptic plasticity, in the mouse hippocampus. The levels of PFDN5 mRNA and protein were increased 2-24h and 4-24h, respectively, after intraperitoneal application of nicotine (3mg/kg, i.p.), finally returning to the basal level. This increase of PFDN5 protein was significantly inhibited by mecamylamine (0.5mg/kg, i.p.), a non-selective nicotinic acetylcholine receptors (nAChRs) antagonist, and required combined application of ABT-418 (10mg/kg, i.p.), a selective alpha4beta2 nAChR agonist, and choline (30mg/kg, i.p.), a selective alpha7 nAChR agonist. In transgenic mice overexpressing human tau with N279K mutation as a model of Alzheimer's disease that showed impaired synaptic plasticity, the levels of PFDN5 mRNA and protein in the hippocampus were significantly decreased in an age-dependent manner as compared with age-matched control. The findings demonstrated that the level of PFDN5 protein in the hippocampus was changed depending on the situation of synaptic plasticity. We propose that PFDN5 could be one of the important components of synaptic plasticity. |
