| First Author | Bellemare V | Year | 2010 |
| Journal | Horm Mol Biol Clin Investig | Volume | 2 |
| Issue | 3 | Pages | 311-8 |
| PubMed ID | 25961203 | Mgi Jnum | J:285858 |
| Mgi Id | MGI:6401034 | Doi | 10.1515/HMBCI.2010.036 |
| Citation | Bellemare V, et al. (2010) Target deletion of the bifunctional type 12 17beta-hydroxysteroid dehydrogenase in mice results in reduction of androgen and estrogen levels in heterozygotes and embryonic lethality in homozygotes. Horm Mol Biol Clin Investig 2(3):311-8 |
| abstractText | 17beta-Hydroxysteroid dehydrogenases (17beta-HSDs) are enzymes issued from convergent evolution of activity from various ancestral genes having different functions. Type 12 17beta-HSD (17beta-HSD12) was described as a bifunctional enzyme, involved in the biosynthesis of estradiol (E2) and the elongation of very long chain fatty acid (VLCFA). It catalyzes selectively the transformation of estrone (E1) into estradiol (E2) in human and primates, whereas in the mouse and Caenorhabditis elegans the enzyme catalyzes the 17beta-reduction of both androgens and estrogens. It is also able to catalyze the reduction of 3-keto-acylCoA into 3-hydroxy-acylCoA in the elongation cycle of VLCFA biosynthesis. To further understand the physiological role of 17beta-HSD12, we performed targeted disruption of the Hsd17b12 gene by substituting exons 8 and 9 that contain the active site with a neomycin cassette. The data indicate that heterozygous (HSD17B12+/-) mice are viable with reduced levels of sex steroids, whereas homozygous (HSD17B12-/-) mice show embryonic lethality. The present data are in agreement with the bifunctional activities of 17beta-HSD12 suggesting that the VLCFA elongation activity, having its origin in the yeast, is most probably responsible for embryonic lethality in HSD17B12-/-, whereas the more recently acquired 17beta-HSD12 activity is responsible for reduced sex steroid levels in HSD17B12+/-. |
