| First Author | Fenton RA | Year | 2006 |
| Journal | Am J Physiol Renal Physiol | Volume | 290 |
| Issue | 1 | Pages | F188-95 |
| PubMed ID | 16091580 | Mgi Jnum | J:286203 |
| Mgi Id | MGI:6402341 | Doi | 10.1152/ajprenal.00285.2005 |
| Citation | Fenton RA, et al. (2006) UT-A urea transporter promoter, UT-Aalpha, targets principal cells of the renal inner medullary collecting duct. Am J Physiol Renal Physiol 290(1):F188-95 |
| abstractText | The urea transporters, UT-A1 and UT-A3, two members of the UT-A gene family, are localized to the terminal portion of the inner medullary collecting duct (IMCD). In this manuscript, we demonstrate that 4.2 kb of the 5'-flanking region of the UT-A gene (UT-Aalpha promoter) is sufficient to drive the IMCD-specific expression of a heterologous reporter gene, beta-galactosidase (beta-Gal), in transgenic mice. RT-PCR, immunoblotting, and immunohistochemistry demonstrate that within the kidney, transgene expression is confined to the terminal portion of the IMCD. Colocalization studies with aquaporin-2 show that expression is localized to the principal cells of the IMCD2 and IMCD3 regions. Utilizing beta-Gal activity assays, we further show that within the kidney, the beta-Gal transgene can be regulated by both water restriction and glucocorticoids, similar to the regulation of the endogenous UT-A gene. These results demonstrate that 4.2 kb of the UT-Aalpha promoter is sufficient to drive expression of a heterologous reporter gene in a tissue-specific and cell-specific fashion in transgenic mice. |
