| First Author | Kara N | Year | 2019 |
| Journal | Cell Rep | Volume | 28 |
| Issue | 8 | Pages | 2037-2047.e4 |
| PubMed ID | 31433981 | Mgi Jnum | J:288447 |
| Mgi Id | MGI:6432251 | Doi | 10.1016/j.celrep.2019.07.061 |
| Citation | Kara N, et al. (2019) The miR-216a-Dot1l Regulatory Axis Is Necessary and Sufficient for Muller Glia Reprogramming during Retina Regeneration. Cell Rep 28(8):2037-2047.e4 |
| abstractText | Unlike the adult mammalian retina, Muller glia (MG) in the adult zebrafish retina are able to dedifferentiate into a "stem cell"-like state and give rise to multipotent progenitor cells upon retinal damage. We show that miR-216a is downregulated in MG after constant intense light lesioning and that miR-216a suppression is necessary and sufficient for MG dedifferentiation and proliferation during retina regeneration. miR-216a targets the H3K79 methyltransferase Dot1l, which is upregulated in proliferating MG after retinal damage. Loss-of-function experiments show that Dot1l is necessary for MG reprogramming and mediates MG proliferation downstream of miR-216a. We further demonstrate that miR-216a and Dot1l regulate MG-mediated retina regeneration through canonical Wnt signaling. This article reports a regulatory mechanism upstream of Wnt signaling during retina regeneration and provides potential targets for enhancing regeneration in the adult mammalian retina. |
