| First Author | Gonome T | Year | 2019 |
| Journal | Biomed Res Int | Volume | 2019 |
| Pages | 6512195 | PubMed ID | 31828115 |
| Mgi Jnum | J:289952 | Mgi Id | MGI:6435240 |
| Doi | 10.1155/2019/6512195 | Citation | Gonome T, et al. (2019) Excess Glutamate May Cause Dilation of Retinal Blood Vessels in Glutamate/Aspartate Transporter-Deficient Mice. Biomed Res Int 2019:6512195 |
| abstractText | Purpose: To investigate the longitudinal findings of fundus features and spectral-domain optical coherence tomography (SD-OCT) to characterize the morphologic features in a mouse model of defective glutamate/aspartate transporter (GLAST(-/-) mice). Materials and Methods: The fundus findings and SD-OCT images were longitudinally recorded at five time points from postnatal (P) 22 to P156 in GLAST(-/-) mice. As a control wild type, age-matched C57BL/6J mice were employed. The mouse retina was subdivided into five layers, and the thickness of each layer was longitudinally measured by InSight(R) using SD-OCT pictures. The SD-OCT findings were compared with the histologic appearances. The diameter of the retinal blood vessels was measured by the ImageJ(R) software program using SD-OCT images. The data were statistically compared between both age-matched mouse groups. Results: The retinal blood vessels appeared more dilated in GLAST(-/-) mice than in wild-type mice. This tendency was statistically significant at all time points after P44 by analyses using SD-OCT images. The ganglion cell complex (GCC) and outer nuclear layer (ONL) were significantly thinner in GLAST(-/-) mice at all time points after P80 than in the wild-type mice. This tendency was more clearly indicated by SD-OCT than histologic sections. Discussion: In the present study, we found for the first time the dilation of the retinal blood vessels and the thinning of the ONL in GLAST(-/-) mice, in addition to the thinning of the GCC. |
