| First Author | Beumer J | Year | 2020 |
| Journal | Cell | Volume | 181 |
| Issue | 6 | Pages | 1291-1306.e19 |
| PubMed ID | 32407674 | Mgi Jnum | J:296073 |
| Mgi Id | MGI:6441326 | Doi | 10.1016/j.cell.2020.04.036 |
| Citation | Beumer J, et al. (2020) High-Resolution mRNA and Secretome Atlas of Human Enteroendocrine Cells. Cell 181(6):1291-1306.e19 |
| abstractText | Enteroendocrine cells (EECs) sense intestinal content and release hormones to regulate gastrointestinal activity, systemic metabolism, and food intake. Little is known about the molecular make-up of human EEC subtypes and the regulated secretion of individual hormones. Here, we describe an organoid-based platform for functional studies of human EECs. EEC formation is induced in vitro by transient expression of NEUROG3. A set of gut organoids was engineered in which the major hormones are fluorescently tagged. A single-cell mRNA atlas was generated for the different EEC subtypes, and their secreted products were recorded by mass-spectrometry. We note key differences to murine EECs, including hormones, sensory receptors, and transcription factors. Notably, several hormone-like molecules were identified. Inter-EEC communication is exemplified by secretin-induced GLP-1 secretion. Indeed, individual EEC subtypes carry receptors for various EEC hormones. This study provides a rich resource to study human EEC development and function. |
