| First Author | Hu D | Year | 2020 |
| Journal | Genesis | Volume | 58 |
| Issue | 9 | Pages | e23384 |
| PubMed ID | 32618127 | Mgi Jnum | J:298082 |
| Mgi Id | MGI:6456959 | Doi | 10.1002/dvg.23384 |
| Citation | Hu D, et al. (2020) Generation of Pecam1 endothelial specific dual reporter mouse model. Genesis 58(9):e23384 |
| abstractText | Endothelial cells are specialized epithelium lining the interior surface of vessels and play fundamental roles in angiogenesis, vascular permeability, and immune response. To identify endothelial cells in vivo, we constructed a Pecam1(nlacZ-H2B-GFP/+) knock-in mouse model in which the endothelial cells are labeled by nuclear LacZ (nlacZ) expression. When Pecam1(nlacZ-H2B-GFP/+) mice are bred with germline Cre deleter mice, Pecam1(H2B-GFP/+) line is created with native nuclear GFP (H2B-GFP) expression in the endothelium of various organs. This dual reporter mouse provides us with a powerful genetic tool for definitive identification of endothelial cells and monitoring this important cell population throughout development, homeostasis, and disease conditions in mammals. |
