| First Author | Hung TY | Year | 2019 |
| Journal | Mol Neurobiol | Volume | 56 |
| Issue | 8 | Pages | 5497-5506 |
| PubMed ID | 30623373 | Mgi Jnum | J:295099 |
| Mgi Id | MGI:6459641 | Doi | 10.1007/s12035-018-1457-2 |
| Citation | Hung TY, et al. (2019) The Protective Role of Peroxisome Proliferator-Activated Receptor-Gamma in Seizure and Neuronal Excitotoxicity. Mol Neurobiol 56(8):5497-5506 |
| abstractText | The peroxisome proliferator-activated receptor (PPAR) family, type II nucleus receptors have been successfully tested for their neuroprotective potential in certain central nervous system diseases. The aim of the present study was to determine if modulation by PPAR-gamma could attenuate pilocarpine-induced seizures and decrease neuronal excitability. Adult male C57BL/6 mice were divided into two groups: one group received pretreatment with pioglitazone and the other received dimethyl sulfoxide (DMSO) for a period of 2 weeks. Status epilepticus was then induced in both groups by lithium-pilocarpine, after which seizure susceptibility, severity, and mortality were evaluated. Hippocampal histopathology was carried out on all mice at 24 h post-status epilepticus as well as blood-brain barrier (BBB) damage analysis. With the aid of patch clamp technology, the hippocampal neuronal excitability from mice with PPAR-gamma 50% expression (Pparg(C/C)) and PPAR-gamma 25% expression (Pparg(C/-)), as well as the effect of pioglitazone on the sodium currents in hippocampal neurons, were evaluated. It was found that pioglitazone, a PPAR-gamma agonist, could attenuate pilocarpine-induced seizure severity in mice. Pathological examination showed that pioglitazone significantly attenuated pilocarpine-induced status epilepticus-related hippocampal neuronal loss and BBB damage. Further characterization of neuronal excitability revealed higher excitability in the brain slices from mice with Pparg(C/-) expression, compared with the Pparg(C/C) group. It was also found that pioglitazone could decrease sodium currents in hippocampal neurons. In conclusion, PPAR-gamma deficiency aggravated neuronal excitability and excitotoxicity. PPAR-gamma attenuated pilocarpine-induced seizure severity, neuronal loss, BBB damage, and sodium currents in hippocampal neurons. Modulation of PPAR-gamma could be a potential novel treatment for epileptic seizures. |
