| First Author | Marinho TS | Year | 2020 |
| Journal | Mol Cell Endocrinol | Volume | 513 |
| Pages | 110872 | PubMed ID | 32454192 |
| Mgi Jnum | J:296878 | Mgi Id | MGI:6469214 |
| Doi | 10.1016/j.mce.2020.110872 | Citation | Marinho TS, et al. (2020) Browning of the subcutaneous adipocytes in diet-induced obese mouse submitted to intermittent fasting. Mol Cell Endocrinol 513:110872 |
| abstractText | PURPOSE: We studied subcutaneous white adipose tissue (sWAT) of obese mice submitted to intermittent fasting (IF). METHODS: Twelve-week-old C57BL/6 male mice received the diets Control (C) or high-fat (HF) for eight weeks (n = 20/each). Then, part of each group performed IF (24 h feeding/24 h fasting) for four weeks: C, C-IF, HF, and HF-IF (n = 10/each). RESULTS: Food intake did not show a difference in feeding and fasting days, but HF groups had a high energy intake. IF led to multilocular adipocytes in sWAT (browning), and improved respiratory quotient on the fed day. IF decreased gene expression of Leptin, but increased Adiponectin, beta3ar (beta3 adrenoreceptor), and Ucp1 (uncoupling protein). IF enhanced immunostaining of Caspase 3, Pcna (proliferating cell nuclear antigen), and UCP1 in sWAT. IF attenuated pro-inflammatory markers and pro-apoptotic markers in sWAT. CONCLUSIONS: IF in obese mice led to browning in sWAT adipocytes, enhanced thermogenesis, an improved adipose tissue pro-inflammatory profile. |
