| First Author | Li Y | Year | 2020 |
| Journal | Mol Cell Endocrinol | Volume | 515 |
| Pages | 110921 | PubMed ID | 32615283 |
| Mgi Jnum | J:296883 | Mgi Id | MGI:6469234 |
| Doi | 10.1016/j.mce.2020.110921 | Citation | Li Y, et al. (2020) GLP-1 promotes osteogenic differentiation of human ADSCs via the Wnt/GSK-3beta/beta-catenin pathway. Mol Cell Endocrinol 515:110921 |
| abstractText | Glucagon-like peptide-1 (GLP-1) analogues are promising anti-diabetic drugs which had been shown to have beneficial effects on bone metabolism in clinical practice, but the molecular mechanism remains unclear. In this study, we evaluated whether GLP-1 can affect the "intestine-fat-bone axis" via the Wnt/GSK-3beta/beta-catenin pathway. We established a diabetic mouse model and then treated mice with GLP-1 analogue liraglutide. The results showed that after liraglutide treatment, glucose tolerance and insulin tolerance were significantly improved in diabetic mice as expected. Moreover, osteogenic markers such as collagen, Runx2 and OCN were upregulated; and the adipogenic differentiation markers C/EBP-alpha and PPAR-gamma were downregulated, these results indicated that liraglutide could ameliorate the osteogenic metabolism in diabetic mice. In the cell model, human ADSCs (hADSCs) were cultured and induced to undergo osteogenic and adipogenic differentiation under high glucose conditions in vitro and then treated with GLP-1. The results showed that GLP-1 repressed the induction of adipocyte differentiation biomarkers and the secretion of GSK-3beta in a dose-dependent manner. In addition, GLP-1 enhanced the expression of osteoblastogenic biomarkers, such as OCN, Runx2 and collagen, and promoted osteoblastic mineralization. These effects were substantially suppressed by the Wnt signal recombinant human DKK-1 or activated by Wnt pathway agonist LiCl. Silencing of GSK-3beta showed that the levels of beta-catenin, GSK-3beta and Runx2 were significantly increased by 2.46-, 2.05-, 4.44-fold after GLP-1 treatment compared to that observed in the GSK-3beta lentiviral group, respectively. We conclude that GLP-1 promotes the osteogenic differentiation of hADSCs via the Wnt/GSK-3beta/beta-catenin pathway. |
