| First Author | Takechi M | Year | 2020 |
| Journal | Biochim Biophys Acta Gen Subj | Volume | 1864 |
| Issue | 10 | Pages | 129679 |
| PubMed ID | 32623025 | Mgi Jnum | J:296890 |
| Mgi Id | MGI:6471458 | Doi | 10.1016/j.bbagen.2020.129679 |
| Citation | Takechi M, et al. (2020) A pericellular hyaluronan matrix is required for the morphological maturation of cortical neurons. Biochim Biophys Acta Gen Subj 1864(10):129679 |
| abstractText | BACKGROUND: Hyaluronan (HA) is a major component of the extracellular matrix (ECM) and is involved in many cellular functions. In the adult brain, HA forms macromolecular aggregates around synapses and plays important roles in neural plasticity. In contrast to the well-characterized function of HA in the adult brain, its roles in the developing brain remain largely unknown. METHODS: Biochemical and histochemical analyses were performed to analyze the amount, solubility, and localization of HA in the developing mouse brain. By combining in utero labeling, cell isolation, and in vitro cultures, we examined the expression of hyaluronan synthase (HAS) and morphological maturation of cortical neurons. RESULTS: The amount of HA increased during perinatal development and decreased in the adult. HA existed as a soluble form in the early stages; however, its solubility markedly decreased during postnatal development. HA localized in cell-sparse regions in the embryonic stages, but was broadly distributed during the postnatal development of the cerebral cortex. Developing cortical neurons expressed both Has2 and Has3, but not Has1, suggesting the autonomous production of HA by neurons themselves. HA formed a pericellular matrix around the cell bodies and neurites of developing cortical neurons, and the inhibition of HA synthesis reduced neurite outgrowth. CONCLUSION: The formation of the pericellular HA matrix is essential for the proper morphological maturation of developing neurons. GENERAL SIGNIFICANCE: This study provides new insights into the roles of hyaluronan in the brain. DEVELOPMENT: |
