| First Author | Rawangwong A | Year | 2020 |
| Journal | Acta Histochem | Volume | 122 |
| Issue | 2 | Pages | 151497 |
| PubMed ID | 31898936 | Mgi Jnum | J:298557 |
| Mgi Id | MGI:6480250 | Doi | 10.1016/j.acthis.2019.151497 |
| Citation | Rawangwong A, et al. (2020) Expression and localization of endogenous phospholipase Cbeta3 confined to basal cells in situ of immature ducts and adult excretory ducts of submandibular gland of mice. Acta Histochem 122(2):151497 |
| abstractText | Our previous study demonstrated that, different from the parotid and sublingual glands, the submandibular glands of adult mice did not show an immunoblot band for PLCbeta3 which is critical in the secretion mechanism by muscarinic cholinergic signaling. Therefore, the submandibular glands of mice at various stages of postnatal development were examined for this enzyme molecule in immunoblot and immunohistochemistry. In immunoblot, a weak band for PLCbeta3-expression was detected only at early postnatal stages. In immunohistochemistry, PLCbeta3-immunoreactivity was distinctly found in most basally located cells of immature ducts, while the immunoreactivity was weakly seen in terminal tubule cells without significant immunoreactivity in adjacent acinar cells. In contrast, the immunoreactivity was distinctly found in some basal cells of adult excretory ducts, and it was ultrastructurally localized densely in close association with bundles of tonofilaments in the cells. The present finding suggests the possibility that Ca(2+) signaling governed by phospholipase Cbeta3 is involved in the differentiation of ductal basal cells into apical cells through control of keratin molecule(s) in the cells. |
