| First Author | Yin C | Year | 2020 |
| Journal | Neuroscience | Volume | 440 |
| Pages | 267-276 | PubMed ID | 32531472 |
| Mgi Jnum | J:298659 | Mgi Id | MGI:6478563 |
| Doi | 10.1016/j.neuroscience.2020.06.004 | Citation | Yin C, et al. (2020) Two Types of Cl Transporters Contribute to the Regulation of Intracellular Cl Concentrations in ON- and OFF-type Bipolar Cells in the Mouse Retina. Neuroscience 440:267-276 |
| abstractText | In the retina, ON- and OFF-type bipolar cells are classified by subtype-specific center responses, which are attributed to differences in glutamate receptor subtypes. However, the mechanisms by which ON- and OFF-type bipolar cells generate subtype-specific surround responses remain unclear. One hypothesis for surround responses is that intracellular Cl concentrations ([Cl-]i) are set at different levels to achieve opposite polarities for GABA responses in ON- and OFF-type bipolar cells. Although this hypothesis is supported by previous findings obtained from rod (ON-) type bipolar cells, there is currently no information on OFF-type bipolar cells. In the present study, we examined the distribution and function of the Cl transporters, the Na-K-Cl co-transporter (NKCC1) and K-Cl co-transporter (KCC2), in rod (ON-) and OFF-type bipolar cells using immunohistochemical, in situ hybridization, and electrophysiological methods. Rod (ON-) and OFF-type bipolar cells both expressed NKCC1 and KCC2. However, the functional contribution of NKCC1 and KCC2 to the regulation of [Cl(-)]i differed between rod (ON-) and OFF-type bipolar cells. Strong NKCC1 activity increased [Cl(-)]i in rod (ON-) type bipolar cells, while that of KCC2 decreased [Cl(-)]i in OFF-type bipolar cells. We also confirmed the presence of a [Cl(-)]i gradient between dendrites and axon terminals in rod (ON-type) bipolar cells. Thus, the subtype-specific control of [Cl(-)]i is achieved by the activity of NKCC1 relative to that of KCC2 and appears to influence the polarity of surround responses. |
