| First Author | Higuchi Y | Year | 2014 |
| Journal | Exp Anim | Volume | 63 |
| Issue | 1 | Pages | 55-62 |
| PubMed ID | 24521863 | Mgi Jnum | J:303453 |
| Mgi Id | MGI:6512212 | Doi | 10.1538/expanim.63.55 |
| Citation | Higuchi Y, et al. (2014) Novel enhanced green fluorescent protein-expressing NOG mouse for analyzing the microenvironment of xenograft tissues. Exp Anim 63(1):55-62 |
| abstractText | The interaction between transplanted cells and host tissues is important for the growth and maintenance of transplanted cells. To analyze the mechanisms of these interactions, a systemic fluorescent protein-expressing mouse is a useful recipient. In this study, we generated a novel NOG strain, which strongly expresses enhanced green fluorescent protein (EGFP; PgkEGFP-NOG), especially in the liver, kidney, gastrointestinal tract, and testis. Because the host tissues expressed EGFP, xenotransplanted human cancer cells were clearly identified as EGFP-negative colonies in PgkEGFP-NOG mice. Immunohistochemical analysis revealed that EGFP-expressing stromal tissues formed a complicated tumor microenvironment within xenograft tissues. Moreover, a similar microenvironment was observed in human iPS cell-derived teratomas. Collectively, these results indicated that a suitable microenvironment is essential for the growth and maintenance of xenotransplanted cells and that PgkEGFP-NOG mice represent a useful animal model for analyzing the mechanisms of microenvironment formation. |
