| First Author | Rossner MJ | Year | 2014 |
| Journal | Brain Res | Volume | 1575 |
| Pages | 1-11 | PubMed ID | 24887641 |
| Mgi Jnum | J:302691 | Mgi Id | MGI:6509376 |
| Doi | 10.1016/j.brainres.2014.05.036 | Citation | Rossner MJ, et al. (2014) Thy1.2 driven expression of transgenic His(6)-SUMO2 in the brain of mice alters a restricted set of genes. Brain Res 1575:1-11 |
| abstractText | Protein SUMOylation is a post-translational protein modification with a key regulatory role in nerve cell development and function, but its function in mammals in vivo has only been studied cursorily. We generated two new transgenic mouse lines that express His6-tagged SUMO1 and SUMO2 driven by the Thy1.2 promoter. The brains of mice of the two lines express transgenic His6-SUMO peptides and conjugate them to substrates in vivo but cytoarchitecture and synaptic organization of adult transgenic mouse brains are indistinguishable from the wild-type situation. We investigated the impact of transgenic SUMO expression on gene transcription in the hippocampus by performing genome wide analyses using microarrays. Surprisingly, no changes were observed in Thy1.2::His6-SUMO1 transgenic mice and only a restricted set of genes were upregulated in Thy1.2::His6-SUMO2 mice. Among these, Penk1 (Preproenkephalin 1), which encodes Met-enkephalin neuropeptides, showed the highest degree of alteration. Accordingly, a significant increase in Met-enkephalin peptide levels in the hippocampus of Thy1.2::His6-SUMO2 was detected, but the expression levels and cellular localization of Met-enkephalin receptors were not changed. Thus, transgenic neuronal expression of His6-SUMO1 or His6-SUMO2 only induces very minor phenotypical changes in mice. |
