| First Author | Muradova E | Year | 2021 |
| Journal | J Invest Dermatol | Volume | 141 |
| Issue | 1 | Pages | 124-131.e2 |
| PubMed ID | 32553564 | Mgi Jnum | J:300331 |
| Mgi Id | MGI:6491518 | Doi | 10.1016/j.jid.2020.05.093 |
| Citation | Muradova E, et al. (2021) Noninvasive Assessment of Epidermal Genomic Markers of UV Exposure in Skin. J Invest Dermatol 141(1):124-131.e2 |
| abstractText | The measurement of UV-induced DNA damage as a dosimeter of exposure and predictor of skin cancer risk has been proposed by multiple groups. Although UV-induced mutations and adducts are present in normal-appearing UV-exposed epidermis, sampling normal nonlesional skin requires noninvasive methods to extract epidermal DNA for analysis. Here, we demonstrate the feasibility of such an approach, termed surfactant-based tissue acquisition for molecular profiling. Sampling in patients was performed using a felt-tip pen soaked in a mixture of surfactants (Brij-30/N-decyl-N,N-dimethyl-3-ammonio-1-propanesulfonate). In mice, we show that the epidermis can be selectively removed without scarring, with complete healing within 2 weeks. We exposed hairless mice to low-dose UV radiation over a period of 3 months and serially sampled them through up to 2 months following the cessation of UV exposure, observing a progressive increase in a UV signature mutational burden. To test whether surfactant-based tissue acquisition for molecular profiling could be applied to human patients, samples were collected from sun-exposed and sun-protected areas, which were then subjected to high-depth targeted exome sequencing. Extensive UV-driven mosaicism and substantially increased mutational loads in sun-exposed versus sun-protected areas were observed, suggesting that genomic measures, as an integrated readout of DNA damage, repair, and clonal expansion, may be informative markers of UV exposure. |
