| First Author | Blumenfeld B | Year | 2021 |
| Journal | Nat Commun | Volume | 12 |
| Issue | 1 | Pages | 1035 |
| PubMed ID | 33589603 | Mgi Jnum | J:304625 |
| Mgi Id | MGI:6515025 | Doi | 10.1038/s41467-021-21348-4 |
| Citation | Blumenfeld B, et al. (2021) Chromosomal coordination and differential structure of asynchronous replicating regions. Nat Commun 12(1):1035 |
| abstractText | Stochastic asynchronous replication timing (AS-RT) is a phenomenon in which the time of replication of each allele is different, and the identity of the early allele varies between cells. By taking advantage of stable clonal pre-B cell populations derived from C57BL6/Castaneous mice, we have mapped the genome-wide AS-RT loci, independently of genetic differences. These regions are characterized by differential chromatin accessibility, mono-allelic expression and include new gene families involved in specifying cell identity. By combining population level mapping with single cell FISH, our data reveal the existence of a novel regulatory program that coordinates a fixed relationship between AS-RT regions on any given chromosome, with some loci set to replicate in a parallel and others set in the anti-parallel orientation. Our results show that AS-RT is a highly regulated epigenetic mark established during early embryogenesis that may be used for facilitating the programming of mono-allelic choice throughout development. |
