| First Author | Chen X | Year | 2021 |
| Journal | Cell Rep | Volume | 34 |
| Issue | 1 | Pages | 108584 |
| PubMed ID | 33406422 | Mgi Jnum | J:303644 |
| Mgi Id | MGI:6690328 | Doi | 10.1016/j.celrep.2020.108584 |
| Citation | Chen X, et al. (2021) linc-AAM Facilitates Gene Expression Contributing to Macrophage Activation and Adaptive Immune Responses. Cell Rep 34(1):108584 |
| abstractText | Although various long noncoding RNAs (lncRNAs) are specifically expressed in activated macrophages, their in vivo functions and mechanisms of action are largely unexplored. Here, we identify a long intergenic noncoding RNA associated with activated macrophage (linc-AAM) and elucidate its function and mechanisms. linc-AAM is highly expressed in activated macrophages. In vitro function analysis reveals that linc-AAM facilitates macrophage activation and promotes the expression of immune response genes (IRGs). In mechanisms, linc-AAM interacts with heterogeneous nuclear ribonucleoprotein L (hnRNPL) via two CACACA motifs, resulting in its dissociation from histone H3 to activate chromatin and facilitate transcription of IRGs. Of note, linc-AAM knockout (KO) mice manifest impaired antigen-specific cellular and humoral immune responses to ovalbumin (OVA) in vivo. Altogether, the results uncover a mechanism of lncRNA in modulating hnRNPL function and confirm that linc-AAM acts as a transcription enhancer to activate macrophages and promote adaptive immunity. |
