| First Author | De Clercq K | Year | 2021 |
| Journal | Cell Mol Life Sci | Volume | 78 |
| Issue | 11 | Pages | 4993-5014 |
| PubMed ID | 33884443 | Mgi Jnum | J:307092 |
| Mgi Id | MGI:6718741 | Doi | 10.1007/s00018-021-03837-3 |
| Citation | De Clercq K, et al. (2021) Mapping the expression of transient receptor potential channels across murine placental development. Cell Mol Life Sci 78(11):4993-5014 |
| abstractText | Transient receptor potential (TRP) channels play prominent roles in ion homeostasis by their ability to control cation influx. Mouse placentation is governed by the processes of trophoblast proliferation, invasion, differentiation, and fusion, all of which require calcium signaling. Although certain TRP channels have been shown to contribute to maternal-fetal transport of magnesium and calcium, a role for TRP channels in specific trophoblast functions has been disregarded. Using qRT-PCR and in situ hybridisation, the spatio-temporal expression pattern of TRP channels in the mouse placenta across gestation (E10.5-E18.5) was assessed. Prominent expression was observed for Trpv2, Trpm6, and Trpm7. Calcium microfluorimetry in primary trophoblast cells isolated at E14.5 of gestation further revealed the functional activity of TRPV2 and TRPM7. Finally, comparing TRP channels expression in mouse trophoblast stem cells (mTSCs) and mouse embryonic stem cells (mESC) confirmed the specific expression of TRPV2 during placental development. Moreover, TRP channel expression was similar in mTSCs compared to primary trophoblasts and validate mTSC as a model to study TRP channels in placental development. Collectivity, our results identify a specific spatio-temporal TRP channel expression pattern in trophoblasts, suggesting a possible involvement in regulating the process of placentation. |
