| First Author | Stipursky J | Year | 2014 |
| Journal | Front Cell Neurosci | Volume | 8 |
| Pages | 393 | PubMed ID | 25484855 |
| Mgi Jnum | J:312336 | Mgi Id | MGI:6783796 |
| Doi | 10.3389/fncel.2014.00393 | Citation | Stipursky J, et al. (2014) TGF-beta1 promotes cerebral cortex radial glia-astrocyte differentiation in vivo. Front Cell Neurosci 8:393 |
| abstractText | The major neural stem cell population in the developing cerebral cortex is composed of the radial glial cells, which generate glial cells and neurons. The mechanisms that modulate the maintenance of the radial glia (RG) stem cell phenotype, or its differentiation, are not yet completely understood. We previously demonstrated that the transforming growth factor-beta1 (TGF-beta1) promotes RG differentiation into astrocytes in vitro (Glia 2007; 55:1023-33) through activation of multiple canonical and non-canonical signaling pathways (Dev Neurosci 2012; 34:68-81). However, it remains unknown if TGF-beta1 acts in RG-astrocyte differentiation in vivo. Here, we addressed the astrogliogenesis induced by TGF-beta1 by using the intraventricular in utero injection in vivo approach. We show that injection of TGF-beta1 in the lateral ventricles of E14,5 mice embryos resulted in RG fibers disorganization and premature gliogenesis, evidenced by appearance of GFAP positive cells in the cortical wall. These events were followed by decreased numbers of neurons in the cortical plate (CP). Together, we also described that TGF-beta1 actions are region-dependent, once RG cells from dorsal region of the cerebral cortex demonstrated to be more responsive to this cytokine compared with RG from lateral cortex either in vitro as well as in vivo. Our work demonstrated that TGF-beta1 is a critical cytokine that regulates RG fate decision and differentiation into astrocytes in vitro and in vivo. We also suggest that RG cells are heterogeneous population that acts as distinct targets of TGF-beta1 during cerebral cortex development. |
