| First Author | Makabe K | Year | 2021 |
| Journal | Int J Mol Sci | Volume | 22 |
| Issue | 7 | PubMed ID | 33806238 |
| Mgi Jnum | J:322064 | Mgi Id | MGI:6751193 |
| Doi | 10.3390/ijms22073396 | Citation | Makabe K, et al. (2021) Dynamics of Cyclooxygenase-1 Positive Microglia/Macrophage in the Retina of Pathological Model Mice as a Biomarker of the Retinal Inflammatory Diseases. Int J Mol Sci 22(7) |
| abstractText | In an intraocular inflammatory state, microglia residing in the retina become active and migrate inside the retina. In this study, we investigated whether cyclooxygenase-1 (COX-1) expressed by retinal microglia/macrophage can be a biomarker for the diagnosis of retinal diseases. COX-1 was immunopositive in microglia/macrophage and neutrophils, while COX-2 was immunopositive in astrocytes and neurons in the inner layer of normal retina. The number of COX-1 positive cells per section of the retinal tissue was 14 +/- 2.8 (mean +/- standard deviation) in normal mice, which showed significant increase in the lipopolysaccharide (LPS)-administrated model (62 +/- 5.0, p = 8.7 x 10(-9)). In addition to microglia, we found neutrophils that were positive for COX-1. In the early stage of inflammation in the experimental autoimmune uveoretinitis (EAU), COX-1 positive cells, infiltrating from the ciliary body into the retinal outer nuclear layer, were observed. The number of infiltrating COX-1 positive cells correlated with the severity of EAU. Taken together, the increased number of COX-1 positive microglia/macrophage with morphological changes were observed in the retinas of retinal inflammatory disease models. This suggests that COX-1 can be a marker of disease-related activities of microglia/macrophage, which should be useful for the diagnosis of retinal diseases. |
