| First Author | Hueso M | Year | 2022 |
| Journal | Biomed Pharmacother | Volume | 146 |
| Pages | 112596 | PubMed ID | 35062066 |
| Mgi Jnum | J:319770 | Mgi Id | MGI:6865280 |
| Doi | 10.1016/j.biopha.2021.112596 | Citation | Hueso M, et al. (2022) MiR-125b downregulates macrophage scavenger receptor type B1 and reverse cholesterol transport. Biomed Pharmacother 146:112596 |
| abstractText | OBJECTIVE: To determine whether miR-125b regulates cholesterol efflux in vivo and in vitro through the regulation of scavenger receptor type B1 (SR-B1). APPROACH AND RESULTS: We demonstrated that miR-125b is up-regulated in the human aortas of patients with CAD and is located in macrophages and vascular smooth muscle cells (VSMCs). We identified SCARB1 as a direct target of miR-125b by repressing the activity of the SCARB1 3'-untranslated region reporter construct. Moreover, the overexpression of miR-125b in both human and mouse macrophages as well as VSMCs was found to downregulated the expression of the SCARB1 and the SR-B1 protein levels, thereby impairing alpha-HDL-mediated macrophage cholesterol efflux in vitro. The in vivo reverse cholesterol transport (RCT) rate from non-cholesterol-loaded macrophages transfected with miR-125b to feces was also found to be decreased when compared with that of control mimic-transfected macrophages. CONCLUSIONS: Together, these results provide evidence that miR-125b downregulates SCARB1 and SR-B1 in both human and mouse macrophages as well as VSMCs, thereby impairing macrophage cholesterol efflux in vitro and the whole macrophage-specific RCT pathway in vivo. |
