| First Author | Gomes RS | Year | 2022 |
| Journal | Cell Immunol | Volume | 371 |
| Pages | 104449 | PubMed ID | 34784560 |
| Mgi Jnum | J:319779 | Mgi Id | MGI:6865389 |
| Doi | 10.1016/j.cellimm.2021.104449 | Citation | Gomes RS, et al. (2022) Protective immune response mediated by neutrophils in experimental visceral leishmaniasis is enhanced by IL-32gamma. Cell Immunol 371:104449 |
| abstractText | Neutrophils are important cells in protection against microbial infections including visceral leishmaniasis (VL). It is well known that IL-32gamma increases the protective T helper 17 cell mediated immune response against Leishmania infantum. Thus, in this study we evaluated whether IL-32 gamma can increase the protective role of neutrophils against VL. In comparison with wild type (WT) mice, transgenic mice for human IL-32 gamma (IL-32 gamma Tg) presented a higher frequency and absolute number of neutrophils in both spleen and liver after the establishment of L. infantum infection. The IL-32 concentrations correlated with neutrophil numbers in the infected tissues. The IL-32 gamma -induced recruitment of neutrophils was dependent on IL-17, since inhibition of Th17 T cells generation and IL-17 production with digoxin treatment reversed the effects of IL-32 gamma. In murine neutrophils, the presence of IL-32 gamma enhanced the phagocytosis of L. infantum via CR3. In addition, murine IL-32 gamma Tg neutrophils were able to kill L. infantum due to the increased production of ROS when compared with WT neutrophils. In fact, IL-32 gamma Tg mice lost their ability to control infection by L. infantum when neutrophils were depleted. In parallel, treatment of human neutrophils with recombinant IL-32 gamma increased phagocytosis and ROS-dependent killing of L. infantum, similarly to murine IL-32 gamma Tg neutrophils. The data show that IL-32 gamma induces neutrophil recruitment to organs affected by VL and increases phagocytosis and killing of L. infantum by neutrophils. Together, data indicate the pivotal axis IL-32 gamma -Th17-neutrophils to control VL. |
