| First Author | Cecchini K | Year | 2023 |
| Journal | Reproduction | Volume | 165 |
| Issue | 2 | Pages | 183-196 |
| PubMed ID | 36395073 | Mgi Jnum | J:344789 |
| Mgi Id | MGI:7414266 | Doi | 10.1530/REP-22-0355 |
| Citation | Cecchini K, et al. (2023) The transcription factor TCFL5 responds to A-MYB to elaborate the male meiotic program in mice. Reproduction 165(2):183-196 |
| abstractText | IN BRIEF: The testis-specific transcription factor, TCFL5, expressed in pachytene spermatocytes regulates the meiotic gene expression program in collaboration with the transcription factor A-MYB. ABSTRACT: In male mice, the transcription factors STRA8 and MEISON initiate meiosis I. We report that STRA8/MEISON activates the transcription factors A-MYB and TCFL5, which together reprogram gene expression after spermatogonia enter into meiosis. TCFL5 promotes the transcription of genes required for meiosis, mRNA turnover, miR-34/449 production, meiotic exit, and spermiogenesis. This transcriptional architecture is conserved in rhesus macaque, suggesting TCFL5 plays a central role in meiosis and spermiogenesis in placental mammals. Tcfl5em1/em1 mutants are sterile, and spermatogenesis arrests at the mid- or late-pachytene stage of meiosis. Moreover, Tcfl5+/em1 mutants produce fewer motile sperm. |
