| First Author | Mizutani K | Year | 2004 |
| Journal | Biochem Biophys Res Commun | Volume | 313 |
| Issue | 3 | Pages | 468-74 |
| PubMed ID | 14697212 | Mgi Jnum | J:386024 |
| Mgi Id | MGI:8346035 | Doi | 10.1016/j.bbrc.2003.11.139 |
| Citation | Mizutani K, et al. (2004) FBP11 regulates nuclear localization of N-WASP and inhibits N-WASP-dependent microspike formation. Biochem Biophys Res Commun 313(3):468-74 |
| abstractText | WASP family proteins are involved in cortical actin cytoskeleton reorganization. Neural Wiskott-Aldrich syndrome protein (N-WASP), a ubiquitously expressed WASP homologous protein, directly binds with Cdc42, activating Arp2/3 complex. In this study, we show that N-WASP-dependent microspike formation is inhibited by formin binding protein 11 (FBP11). Endogenous FBP11 localizes with nuclear-speckles, and co-localization of N-WASP and FBP11 was observed when they were co-expressed. Epidermal growth factor (EGF) induced actin-microspike formation in COS7 cells. However, transient expression of FBP11 suppressed N-WASP-dependent actin-microspike formation by trapping N-WASP in the nucleus. These results indicate that FBP11 regulates localization of N-WASP, thus negatively regulating the function of N-WASP in the cytoplasm. |
