| First Author | Yonezawa S | Year | 1998 |
| Journal | Biomed Res | Volume | 19 |
| Issue | 5 | Pages | 327-334 |
| Mgi Jnum | J:55946 | Mgi Id | MGI:1339811 |
| Doi | 10.2220/biomedres.19.327 | Citation | Yonezawa S, et al. (1998) Cathepsin E gene in mouse. Biomed Res 19(5):327-334 |
| abstractText | A 16.8-kb genomic clone containing the mouse cathepsin E gene was isolated from a 129/SvJ lambda Fix II library. The gene was determined to be 13.8 kb in length and to consist of 9 exons. 5'- and 3'-untranslated regions in the clone were 2.5 kb and 0.5 kb in length, respectively. The overall exon/intron organization of the mouse cathepsin E gene was similar to that of the human gene, and highly conserved sequences were identified in the 5'-flanking region containing potential binding motifs for GATA- transcriptional factors. Further upstream, potential MZF-1- binding elements were found in both the mouse and human genes and a (CA)(23) sequence in the mouse gene. Genomic Southern analysis with 5 different restriction enzymes strongly suggested the existence of only a single cathepsin E gene in the mouse. Cathepsin E expression in various mouse tissues was uniform on the analysis with PAGE and anion-exchange chromatography, supporting the single gene hypothesis. The mouse cathepsin E gene was assigned to chromosome 1 band F by fluorescence in situ hybridization. |
