| First Author | Xing L | Year | 2024 |
| Journal | Proc Natl Acad Sci U S A | Volume | 121 |
| Issue | 9 | Pages | e2320276121 |
| PubMed ID | 38381789 | Mgi Jnum | J:358311 |
| Mgi Id | MGI:7609994 | Doi | 10.1073/pnas.2320276121 |
| Citation | Xing L, et al. (2024) Diverse roles of pontine NPS-expressing neurons in sleep regulation. Proc Natl Acad Sci U S A 121(9):e2320276121 |
| abstractText | Neuropeptide S (NPS) was postulated to be a wake-promoting neuropeptide with unknown mechanism, and a mutation in its receptor (NPSR1) causes the short sleep duration trait in humans. We investigated the role of different NPS(+) nuclei in sleep/wake regulation. Loss-of-function and chemogenetic studies revealed that NPS(+) neurons in the parabrachial nucleus (PB) are wake-promoting, whereas peri-locus coeruleus (peri-LC) NPS(+) neurons are not important for sleep/wake modulation. Further, we found that a NPS(+) nucleus in the central gray of the pons (CGPn) strongly promotes sleep. Fiber photometry recordings showed that NPS(+) neurons are wake-active in the CGPn and wake/REM-sleep active in the PB and peri-LC. Blocking NPS-NPSR1 signaling or knockdown of Nps supported the function of the NPS-NPSR1 pathway in sleep/wake regulation. Together, these results reveal that NPS and NPS(+) neurons play dichotomous roles in sleep/wake regulation at both the molecular and circuit levels. |
