| First Author | Neumann E | Year | 2024 |
| Journal | J Neurosci | Volume | 44 |
| Issue | 41 | PubMed ID | 39137998 |
| Mgi Jnum | J:355330 | Mgi Id | MGI:7738162 |
| Doi | 10.1523/JNEUROSCI.0591-24.2024 | Citation | Neumann E, et al. (2024) gamma1 GABA(A) Receptors in Spinal Nociceptive Circuits. J Neurosci 44(41) |
| abstractText | GABAergic neurons and GABA(A) receptors (GABA(A)Rs) are critical elements of almost all neuronal circuits. Most GABA(A)Rs of the CNS are heteropentameric ion channels composed of two alpha, two beta, and one gamma subunits. These receptors serve as important drug targets for benzodiazepine (BDZ) site agonists, which potentiate the action of GABA at GABA(A)Rs. Most GABA(A)R classifications rely on the heterogeneity of the alpha subunit (alpha1-alpha6) included in the receptor complex. Heterogeneity of the gamma subunits (gamma1-gamma3), which mediate synaptic clustering of GABA(A)Rs and contribute, together with alpha subunits, to the benzodiazepine (BDZ) binding site, has gained less attention, mainly because gamma2 subunits greatly outnumber the other gamma subunits in most brain regions. Here, we have investigated a potential role of non-gamma2 GABA(A)Rs in neural circuits of the spinal dorsal horn, a key site of nociceptive processing. Female and male mice were studied. We demonstrate that besides gamma2 subunits, gamma1 subunits are significantly expressed in the spinal dorsal horn, especially in its superficial layers. Unlike global gamma2 subunit deletion, which is lethal, spinal cord-specific loss of gamma2 subunits was well tolerated. GABA(A)R clustering in the superficial dorsal horn remained largely unaffected and antihyperalgesic actions of HZ-166, a nonsedative BDZ site agonist, were partially retained. Our results thus suggest that the superficial dorsal horn harbors functionally relevant amounts of gamma1 subunits that support the synaptic clustering of GABA(A)Rs in this site. They further suggest that gamma1 containing GABA(A)Rs contribute to the spinal control of nociceptive information flow. |
