| First Author | Lin KK | Year | 2004 |
| Journal | Proc Natl Acad Sci U S A | Volume | 101 |
| Issue | 45 | Pages | 15955-60 |
| PubMed ID | 15520371 | Mgi Jnum | J:356487 |
| Mgi Id | MGI:7762579 | Doi | 10.1073/pnas.0407114101 |
| Citation | Lin KK, et al. (2004) Identification of hair cycle-associated genes from time-course gene expression profile data by using replicate variance. Proc Natl Acad Sci U S A 101(45):15955-60 |
| abstractText | The hair-growth cycle is an example of a cyclic process that is well characterized morphologically but understood incompletely at the molecular level. As an initial step in discovering regulators in hair-follicle morphogenesis and cycling, we used DNA microarrays to profile mRNA expression in mouse back skin from eight representative time points. We developed a statistical algorithm to identify the set of genes expressed within skin that are associated specifically with the hair-growth cycle. The methodology takes advantage of higher replicate variance during asynchronous hair cycles in comparison with synchronous cycles. More than one-third of genes with detectable skin expression showed hair-cycle-related changes in expression, suggesting that many more genes may be associated with the hair-growth cycle than have been identified in the literature. By using a probabilistic clustering algorithm for replicated measurements, these genes were grouped into 30 time-course profile clusters, which fall into four major classes. Distinct genetic pathways were characteristic for the different time-course profile clusters, providing insights into the regulation of hair-follicle cycling and suggesting that this approach is useful for identifying hair follicle regulators. In addition to revealing known hair-related genes, we identified genes that were not previously known to be hair cycle-associated and confirmed their temporal and spatial expression patterns during the hair-growth cycle by quantitative real-time PCR and in situ hybridization. The same computational approach should be generally useful for identifying genes associated with cyclic processes from complex tissues. |
