| First Author | Nichane M | Year | 2017 |
| Journal | Nat Methods | Volume | 14 |
| Issue | 12 | Pages | 1205-1212 |
| PubMed ID | 29106405 | Mgi Jnum | J:356776 |
| Mgi Id | MGI:7762868 | Doi | 10.1038/nmeth.4498 |
| Citation | Nichane M, et al. (2017) Isolation and 3D expansion of multipotent Sox9(+) mouse lung progenitors. Nat Methods 14(12):1205-1212 |
| abstractText | Multiple adult tissues are maintained by stem cells of restricted developmental potential which can only form a subset of lineages within the tissue. For instance, the two adult lung epithelial compartments (airways and alveoli) are separately maintained by distinct lineage-restricted stem cells. A challenge has been to obtain multipotent stem cells and/or progenitors that can generate all epithelial cell types of a given tissue. Here we show that mouse Sox9(+) multipotent embryonic lung progenitors can be isolated and expanded long term in 3D culture. Cultured Sox9(+) progenitors transcriptionally resemble their in vivo counterparts and generate both airway and alveolar cell types in vitro. Sox9(+) progenitors that were transplanted into injured adult mouse lungs differentiated into all major airway and alveolar lineages in vivo in a region-appropriate fashion. We propose that a single expandable embryonic lung progenitor population with broader developmental competence may eventually be used as an alternative for region-restricted adult tissue stem cells in regenerative medicine. |
