| First Author | Weberling A | Year | 2025 |
| Journal | iScience | Volume | 28 |
| Issue | 1 | Pages | 111671 |
| PubMed ID | 39868030 | Mgi Jnum | J:361444 |
| Mgi Id | MGI:7858625 | Doi | 10.1016/j.isci.2024.111671 |
| Citation | Weberling A, et al. (2025) Primitive to visceral endoderm maturation is essential for mouse epiblast survival beyond implantation. iScience 28(1):111671 |
| abstractText | The implantation of the mouse blastocyst initiates a complex sequence of tissue remodeling and cell differentiation events required for morphogenesis, during which the extraembryonic primitive endoderm transitions into the visceral endoderm. Through single-cell RNA sequencing of embryos at embryonic day 5.0, shortly after implantation, we reveal that this transition is driven by dynamic signaling activities, notably the upregulation of BMP signaling and a transient increase in Sox7 expression. Embryos deficient in Hepatocyte nuclear factor-1-beta (Hnf1b-/-), a gene critical for visceral endoderm differentiation, showed an interaction between visceral endoderm and epiblast, crucial for epiblast survival. Single-cell RNA profiling of Hnf1b-/- visceral endoderm shows developmental delays and severe dysregulation in several nutrient transport pathways. Impaired glucose uptake in Hnf1b-/- embryos suggests that the activation of nutrient transport mechanisms during the primitive-to-visceral endoderm transition may be vital for post-implantation epiblast development. These findings offer new insights into the molecular regulation of early mammalian development. |
