| First Author | Frankel WN | Year | 1991 |
| Journal | Mouse Genome | Volume | 89 |
| Pages | 266-70 | Mgi Jnum | J:14303 |
| Mgi Id | MGI:62474 | Citation | Frankel WN, et al. (1991) Backcross data for endogenous Mpmv, Pmv, and Xmv proviruses. Mouse Genome 89:266-70 |
| abstractText | Full text of Mouse Genome contribution: Backcross Data for Endogenous Mpmv, Pmv, and Xmv Proviruses. Wayne N. Frankel, John M. Coffin, Matthew Cote; Dep't. Mol. Biology, Tufts Univ. Sch. Med., Boston, MA. Thomas N. Seyfried and Matthew Rise; Dep't. Biology, Boston College, Chestnut Hill, MA. T. V. Rajan and F. Kenneth Nelson; Dep't Pathology, Univ. Ct. Hth Sci. Ctr., Farmington CT. Erik Selsing and Rachel Gerstein; Dep't. Biology, Brandeis Univ., Waltham, MA. Linkage data. In this communication we show BC data for endogenous nonecotropic Mpmv, Pmv, and Xmv proviral loci, previously mapped using RI strains (1, 2, 3). The incentive for this arose from the 4th International Workshop on the Mouse Genome, where chromosome committees' priorities for their "best", genetic maps would be given to BC, then IC, then (when necessary) RI data. Although parts of these BC data are published elsewhere, (4, 5,6, 7), we could not possibly show the most of the data, given the context of those papers. On Tables 1, 2, and 3, BXCXC refers to a (C57BL/6J x BALB/cJ) Fl x BALB/cJ cross, DXlX1 to (DBA/lJ x 129/J)Fl x 129/J, EXAXA to (El x ABP/LeJ)Fl x ABP/LeJ, and EXDXD to (El x DBA/2J)Fl x DBA/2J. Overall, the numbers of Fl males and females are about the same. We scored Mpmv, Pmv, and Xmv 3' junction fragments using oligonucleotide viral env probes (8), and Mtv 5' or 3' junction fragments using the MMTV LTR probe (9). In a BC, fragments were scored on Southern blots of EcoRI and PvuII -digested DNA as plus/minus when unique to the "donor" strain (i.e. El in EXAXA) and by gene dosage for those unique to the recurrent partner. For the latter, confirmatory data was often obtained with both restriction enzymes, and proviruses common to both parents always served as reliable internal homozygous controls. Note that some well-mapped loci were also used Bxv-1, b, Ly-2, wa-1, p, c, d, se, Bgl, Rnr-12, bt, and Rnr-16. In addition, we show mapping data for thirteen novel proviruses in E1 and 129/J mice. Molecular data from proviruses unique to El (Mpmv-43, Mpmv-44, Xmv-60, Xmv-61, Xmv-62, and Xmv-63) will be presented separately (7). The 129/J strain contains Mpmv-40 (EcoRI -3.59 kb, PvuII - 11 kb), Mpmv-41 (EcoRI - 3.7 kb, PvuII - 3.1 kb), Pmv-62 (EcoRI - 2 kb, PvuII - 3.5 kb), Pmv-63 (EcoRI - 3.5 kb, PvuII Ð 2.52 kb), Pmv-65 (EcoRI - 3.13 kb, PvuII - 4.76 kb), Xmv-53 (EcoRI Ð 2.61 kb, PvuII - 3.95 kb), and Mtv-"4.8" (EcoRI - 4.8 kb). In 129/J, Pmv-64 (EcoRI - 3.59 kb, PvuII - 3.85 kb), is not linked to anything scored in the DXlX1 cross, and the unmapped Pmv-61 (EcoRI - 3.4 kb, PvuII - 8.6 kb), cannot be scored in a BC when 129/J is the recurrent partner - due to co-migrating fragments. Tables 1 and 2 provide support for gene order. When possible, multipoint pedigrees are shown (Table 1). Our convention is to show the donor strain chromosome in each cross in black, and that from the partner in white. On Table 1, the column "+ strainÓ indicates which parent contains the provirus. Although the number of doubles may appear high, please note that some of these genetic intervals are quite large. Table 2 shows 3-point cross data for certain loci, mutually exclusive of pedigrees (no overlap). The best gene order is shown in a box, with the "odds" column merely being a relative statement of order (10). Table 3 summarizes 2-point data in each cross. In the "Locus 1" column, in the region enclosed in a box, the best gene order is listed top-bottom for each chromosome. Below the boxed region are additional data for map distances that span flanking markers, when it seemed appropriate, necessary, or when there was room. For the most part, gene orders and map distances for loci shown here agreed with that inferred previously from the RI data (3); however, a few major exceptions to this, as well as assignments for previously unmapped loci, are marked with asterisks. The rest is self explanatory. We hope that these data are useful. For those running multipoint linkage analyses programs, these data are currently stored in Microsoft Excel files which can be converted into text for uploading/ downloading into your system. References: 1. W.N. Frankel, J.P. Stoye, B.A. Taylor, J.M. Coffin, J. Virol. 63,1762-1774 (1989). 2. W.N. Frankel, J.P. Stoye, B.A. Taylor, J.M. Coffin, J. Virol. 63, 3810-3821 (1989). 3. W.N. Frankel, J.P. Stoye, B.A. Taylor, J.M. Coffin, Genetics 124, 221-236 (1990). 4. R.M. Gerstein, et al., Cell 63, 537-548 (1990). 5. F.K. Nelson, W.N. Frankel, T.V. Rajan, Mol. Cell. Biol. 9:1284-1288, (1989). 6. M. Rise, T.N. Seyfried, Mouse Newsletter 86 (February, 1990). 7. M. Rise, W.N. Frankel, J.M. Coffin, T.N, Seyfried, submitted for publication (1990). 8. J.P. Stoye, J.M. Coffin, J. Virol. 62, 168-175 (1988). 9. J.E. Majors, H.E. Varmus. Nature 289:253-258. (1981). 10. D.T. Bishop, Genet. Epidemiol. 2, 349-361 (1985). FAX (WNF, JMC): (617) 956-0337; INTERNET: wfrankel@opal.tufts.edu |
