| First Author | Greenfield AJ | Year | 1992 |
| Journal | Mouse Genome | Volume | 90 |
| Issue | 1 | Pages | 94-95 |
| Mgi Jnum | J:36 | Mgi Id | MGI:48577 |
| Citation | Greenfield AJ, et al. (1992) Mappping of clone D4Smh6b to the distal end of mouse chromosome 4. Mouse Genome 90(1):94-95 |
| abstractText | Full text of Mouse Genome contribution: Mapping of clone D4Smh6b to the distal end of mouse chromosome 4. Andrew J. Greenfieldl Stephen D.M. Brownl, Jeffrey M. Friedman2 & Nathan Bahary2 lDepartment of Biochemistry & Molecular Genetics, St. Mary's Hospital Medical School, London W2 lPG, UK 2Laboratory of Molecular Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021, USA Introduction Clone D4Smh6b was isolated during a screening of a mouse cosmid library with a cDNA probe (ATCC No. 57676) from the human dystrophin genel. This cosmid clone hybridised weakly to the cDNA probe after washing at low stringency (data not shown) and was examined further on the basis of its putative homology to dystrophin sequences. Genetic analysis of D4Smh6b through a panel of interspecific backcross progeny mice segregating for the X chromosome mutation mdx2 indicated an autosomal pattern of inheritance. We present here the segregation analysis of D4Smh6b in a panel of 98 Mus spretus/ C57BL/6J interspecific backcross mice typed for 20 mouse chromosome 4 loci3 and demonstrate that D4Smh6b maps to the distal end of chromosome 4. Materials and Methods The cosmid library, dystrophin cDNA probe and screening procedures utilised are as described in Ref. 1. Generation of interspecific backcross progeny mice, origins of the chromosome 4 markers and restriction/hybridisation methods are as described in Ref. 3. Results A 2.5kb EcoRI subclone from the D4Smh6b cosmid was hybridised to TaqI-digested DNA samples from 98 (C57BL/6J x Mus spretus) x CS7BL/6J backcross progeny mice previously characterised for 20 markers mapping to MMU4(3). The 2.5kb single-copy fragment detects a 3.5kb C57BL/6J TaqI restriction fragment length variant and 3.0kb Mus spretus TaqI variant on genomic digest filters (data not shown). Fig. 1 summarises all of the haplotype data for the segregation of this restriction fragment length variant in the 98 backcross progeny. Discussion The haplotype analysis presented in Fig. 1 indicates that D4Smh6b maps 1.03 +/- 1.03cM (1 recombinant in 97 chromsomes) distal to Pnd. Another marker, Skv, has been reported to map distal to Pnd(4) but the relationship between Skv and D4Smh6b has not been determined. It has also been reported that in situ hybridization experiments indicate that D4Smh6b hybridises quite close to the telomere of chromosome 4(4). D4Smh6b is one of the most distal markers on mouse chromosome 4. In addition, 4 different microsatellite sequences have been characterised from this cosmid(5) making D4Smh6b a particularly useful marker for the joint physical and genetic mapping of the distal end of mouse chromosome 4. References 1. Maconochie, M.K., Brown, S.D.M. & Greenfield, A.J. (1992) Sequence analysis of two exons from the murine dystrophin locus. Mammalian Genome 2: 64-68. 2. Cavanna, J.S., Coulton, G., Morgan, J.E., Brockdorff, N., Forrest, S.M., Davies, K.E. & Brown, S.D.M. (1988) Molecular and Genetic Mapping of the mouse mdx locus. Genomics 3:337-341. 3. Bahary, N., Zorich, G., Pachter, J.E., Leibel, R.L. & Friedman, J.M. (1991) Molecular Genetic Linkage Maps of Mouse Chromosomes 4 and 6. Genomics 11: 33-47. 4. Blank, R., Eppig, J., Fiedorek, F.T., Frankel, W.N., Friedman, J.M., Huppi, K, Jackson, I. & Mock, B. (1991) Mouse Chromosome 4. Mammalian Genome 1: S51-S78. 5. Hearne, C.M., McAleer, M.A., Love, J.M., Aitman, T.J., Cornall, R.J., Ghosh, S., Knight, A.M., Prins, J.-B. & Todd, J.A. (1991) Additional microsatellite markers for mouse genome mapping. Mammalian Genome 1: 273-282. Figure 1. (Legend). a) Haplotype analysis of the 21 chromosome 4 loci (including D4Smh6b) in 83 (C57BL/6J x M. spretus) x C57BL/6J backcross progeny demonstrating no crossover or a single crossover event. b) Haplotype analysis of 15 backcross progeny mice exhibiting double recombination events. (No data for D4Smh6b is available for one of these mice - see below). Mapped loci are listed on the left. The number of progeny with a particular haplotype is shown at the bottom. Blackened boxes represent the M. spretus restriction fragment variant, the white boxes represent the C57BL/6J variant and the diagonally striped boxes represent instances for which no data are available. |
