| First Author | Miller RD | Year | 1994 |
| Journal | Mouse Genome | Volume | 92 |
| Issue | 4 | Pages | 694-695 |
| Mgi Jnum | J:22096 | Mgi Id | MGI:69987 |
| Citation | Miller RD (1994) A backcross panel typed for several immunoglobulin gene superfamily members on MMU9. Mouse Genome 92(4):694-695 |
| abstractText | Full text of Mouse Genome contribution: A backcross panel typed for several immunoglobulin gene superfamily members on MMU9. Robert D. Millerl, Jennifer H. Ozaki, and Roy Riblet. Medical Biology Institute, 11077 North Torrey Pines Road, La Jolla, CA 92037 USA. 1present address: Department of Biology, The University of New Mexico, Albuquerque, NM 87131 USA. Several members of the immunoglobulin gene superfamily, Thy-1, Ncam, CD3g, CD3d, and CD3e are closely linked in a region conserved between mouse chromosome 9 (MMU9) and human chromosome 11q22-q23 (HSA11q22-q23; Kingsley, 1993). CD3g, CD3d, and CD3e are physically close together, spanning a region of less than 50 kb in both species (Evans et al., 1988). We previously reported a linkage map of this region based on typing two Recombinant Inbred (RI) mouse panels (Miller et al., 1989). The best gene order from the RI data was Thy-1- Apoa-1 -CD3d -Ncam. This gene order, however, conflicted with the order Thy-1 - CD3d -Apoa-1-Ncam expected from linkage distances determined on separate backcross panels (Kingsley, 1993) and the location of CD3d between Thy-1 and Apoa-1 on HSA11q22-q23 as reported by Yunis and colleagues (1989). We decided to resolve this conflict by typing a large backcross panel for loci in this region. Genomic DNA was prepared from 135 (BALB/c x CASA/Rk)F1 x BALB/c backcross progeny and 115 BALB/c x (BALB/c x CASA/Rk)F1 backcross progeny. All 250 DNAs were typed for seven loci listed in Figure 1. All but one of these loci were typed by PCR as simple sequence length polymorphisms (SSLP). The SSLPs used are listed in Table 1 along with the relative length difference between BALB/c and CASA/Rk. The Cbl-2 locus was typed as a Restriction Fragment Length Variant by Southern blot hybridization of PvuII digested genomic DNA hybridized with the v-cbl probe (provided by Dr. H.C. Morse, National Institutes of Health, Bethesda, MD, USA). Southern blotting and hybridization conditions were as previously described (Miller et al., 1988). A summary of the results of this typing is shown in Figure 1. No recombinants were found between Cbl-2 and Thy-1 in 250 backcross progeny. These results establish a locus order of centromere - D9Mit1 - D9Mit2 - [Cbl-2, Thy-1] - CD3d - Ncam - Cyp1a2 - telomere. The 24 backcross progeny with recombinations in the interval between Thy-1 and Cyp1a2 were further typed for the Apoa1 locus by Southern blot hybridization of PstI digested DNA using insert DNA from the p1804 clone as a probe (Barth et al., 1982, provided by Dr. Rosemary Elliot, Roswell Park Memorial Institute, Buffalo, NY, USA). A single recombinant found between CD3d and Apoa I placed this locus distal. The locus order for MMU9 presented in Figure 2 is in agreement with recent chromosome committee report on the map of HSA11 (Junien and van Heyningen, 1991). This work was supported by NIH grant AI23548 to R.R.; R.D.M. was a fellow of the Juvenile Diabetes Foundation International Inc. References Aitman T.J., Hearne C.M., McAleer M.A., and Todd J.A. Mammalian Genome 1:206 210, 1991. Barth, R.K., Gross. K.W., Gremke, L.C., and Hastie, N.A. Proc. Natl. Acad. Sci. 79:500-504, 1982. Dietrich, W., Katz, H., Lincoln, S.E., Shin, H-S., Friedman, J., Dracopoli, N., and Lander, E.S. Genetics 131:423-447, 1992. Evans, G.A., Lewis, K.A,, and Lawless, G.M. Immunogenetics 28:365-373, 1988. Hearne C.M., McAleer M.A., Love J.M., Aitman T.J., Cornall R.J., Ghosh S. Knight A.M., Prins J.B., and Todd J.A. Mammalian Genome, 1:273-282, 1991. Junien C. and van Heyningen V. Cytogenet. Cell Genet. 58:459-554, 1991. Kingsley, D. Mammalian Genome 4 :S136-S153, 1993. Love, J.M., Knight, A.M., McAleer, M.A. and Todd, J.A. Nucl. Acids Res. 18:4123-4130, 1990. Manly and Elliott, Mammalian Genome 1:123, 1991. Miller, R.D., Ozaki, J.H., Riblet, R. and Gold, D.P. Immunogenetics: 30:511-514, 1989. Yunis, J. J., Jones, C., Madden, M.T., Lu, D., and Mayer, M.G. Genomics 5:84-90, 1989. Fig. 1. (Legend). Results of DNA typing of backcross progeny. Loci shown have been typed on all 250 backcross progeny. Values at the bottom of the figure are the number of progeny inheriting the indicated chromosome haplotype from the F1 parent. Values to the right of the figure are recombination frequencies. Table 1. SSLP markers used and the relative PCR product size difference between CASA/Rk and BALB/c. Gene/Locus: D9Mit1; Relative size difference: CASA/Rk > BALB/c; Reference: Dietrich et al., 1992. Gene/Locus: D9Mit2; Relative size difference: CASA/Rk > BALB/c; Reference: Dietrich et al., 1992. Gene/Locus: Thy-1; Relative size difference: CASA/Rk > BALB/c; Reference: Aitman et al., 1991 (sequence no. 124). Gene/Locus: CD3d; Relative size difference: CASA/Rk > BALB/c; Reference: Hearn et al., 1991 (sequence no. 112). Gene/Locus: CD3d (D9Mit23); Relative size difference: CASA/Rk > BALB/c; Reference: Dietrich et al., 1992. Gene/Locus: Ncam; Relative size difference: BALB/c > CASA/Rk; Reference: Love et al., 1991 (sequence no. 12). Gene/Locus: CyplA2; Relative size difference: CASA/Rk> BALB/c; Reference: Love et al., 1991 (sequence no. 34). Fig. 2. (Legend). Genetic maps derived from the maternal F1, paternal F1, and combined backcross data. Values to the left of the chromosome are estimated distances in cM calculated using the statistics functions within the RI Manager Ver 2.2 (Manly and Elliott, 1991). |
