| First Author | Naylor SL | Year | 1984 |
| Journal | Cytogenet Cell Genet | Volume | 37 (1-4) |
| Pages | 551 (Abstr.) (399-616) | Mgi Jnum | J:12258 |
| Mgi Id | MGI:60507 | Citation | Naylor SL, et al. (1984) Chromosomal mapping of mouse hormone genes: somatostatin, calcitonin, thyrotropin alpha subunit, and luteinizing hormone beta subunit (Abstracts of meeting presentations: Human gene mapping 7, Los Angeles Conference (1983) Seventh International Workshop on Human Gene Mapping). Cytogenet Cell Genet 37 (1-4):551 (Abstr.) (399-616) |
| abstractText | Full text of Abstract: Abstracts of meeting presentations. ............................... PAGE 551 Chromosomal mapping of mouse hormone genes: somatostatin, calcitonin, thyrotropin alpha subunit, and luteinizing hormone beta subunit. S.L. Naylor1 A.Y. Sakaguchi,1 W.W. Chin,2 J. Jacobs,2 L.P. Shen,3 W.J. Rutter,3 and P.A. Lalley4 1Roswell Park Memorial Institute, Buffalo, NY, 2Massachusetts General Hospital and Harvard Medical School, Boston, MA, 3 University of California, San Francisco, CA, and 4Oak Ridge Laboratories, Oak Ridge, TN. Genes for the hormones somatostatin (SST), calcitonin(CT), thyrotropin alpha subunit (TSH-alpha), and luteinizing hormone beta subunit (LH-beta) have been mapped to mouse chromosomes using mouse- Chinese hamster somatic cell hybrids. DNA was isolated from a panel of 15 mouse-Chinese hamster hybrids, cleaved with a specific restriction endonuclease, and probed with either mouse (TSH-alpha) and rat (CT and LH-beta) cDNA clones or a human genomic clone (SST). The 7.8 kb mouse-HindIII fragment detected by the calcitonin cDNA clone segregated concordantly with mouse chromosome 7. The human somatostatin probe detected a 20 kb fragment in EcoRI cleaved mouse DNA that segregated with chromosome 16 in the hybrid cells. TSH-alpha cDNA hybridized to a single 13.4 kb EcoRI fragment in mouse DNA which in the hybrids co-segregated with chromosome 4. The beta subunit gene of luteinizing hormone was detected a s a 1.5 kb EcoRI fragment which segregated concordantly with mouse chromosome 7. Thus, we have mapped the calcitonin gene to chromosome 7, somatostatin gene (SST) to 16, the thyrotropin alpha subunit (TSH-alpha) to 4, and the luteinizing hormone beta subunit gene (LH-beta) to 7. LH-beta appears as though it may lie in a conserved region since chromosome 7 also has genes for glucose phosphate isomerase and peptidase-4 (D) whose homologous genes as well as the LH-beta gene are on human chromosome 19. |
